DNA finger printing
- DNA fingerprinting is a technique that simultaneously detects lots of minisatellites in the genome to produce a pattern unique to an individual.
- It was developed by Alec Jefferys in 1985.
- It is generally used for the identification of animals from blood strains, semen, etc. and for establishing parentage in case of dispute.
- DNA finger print is essentially a southern blot of DNA digested with an endonuclease and probed with radioactive DNA probe.
DNA Fingerprinting Steps
- Collection of organic example blood, spit, buccal swab, semen, or solid tissue.
- DNA extraction.
- Restriction absorption or PCR intensification.
- Agarose gel electrophoresis, slim electrophoresis or DNA sequencing.
- Interpreting outcomes.
The Process of DNA Fingerprinting
Sample collection, DNA extraction, absorption or intensification and investigation results are significant advances.
Stage 1: Sample Collection
DNA can be acquired from any bodily sample or liquid. Buccal smear, salivation, blood, amniotic liquid, chorionic villi, skin, hair, body liquid, and different tissues are significant kinds of samples utilized.
Stage 2: DNA Extraction
We need to initially get DNA. To play out any genetic applications, DNA extraction is one of the most significant advances. Great quality and amount of DNA expands the conceivable outcomes of getting better outcomes.
You can utilize DNA extraction strategies enrolled beneath,
- Phenol-chloroform DNA extraction strategy
- CTAB DNA extraction strategy
- Proteinase K DNA extraction strategy
In any case, we emphatically prescribe utilizing a ready to go DNA extraction unit for DNA fingerprinting.
The immaculateness and amount of DNA ought to be ~1.80 and 100ng, individually to play out the DNA test. Filter the DNA utilizing the DNA sanitization unit, if necessary.
From that point onward, measure the DNA utilizing the UV-Visible spectrophotometer. Furthermore, perform one of the accompanying strategies recorded underneath.
Stage 3: Restriction Absorption, Enhancement or DNA Sequencing
Three regular strategies are utilized:
- RFLP based STR investigation
- PCR based investigation
- Real-time PCR investigation
- Stage 4: Analysis of Results
As we examined, utilizing the southern blotting, agarose gel electrophoresis, narrow electrophoresis, ongoing intensification, and DNA sequencing, the outcomes for different DNA profiling can be gotten in which rt-PCR and sequencing are much of the use in forensic science.
- Stage 5: Interpreting Results
By looking at DNA profiles of different examples, varieties and likenesses between people can be distinguished. Outstandingly, the whole procedure is presently nearly automatic. We don’t need to do anything, the computer gives us conclusive outcomes.