Anti-Coagulants used in Blood Collection

Ammonium and Potassium oxalate:

• Proportion of ammonium oxalate to potassium oxalate should be 60:40
• It is known as Heller and Paul’s mixture
• 2 g of ammonium oxalate and 0.8 g of potassium oxalate are taken.

100 ml of distilled water is then added to mixture——-> 0.5 ml of this solution are taken in specimen tube——–> Solution are then incubated at 70°C. this mixture is adequate for 5 ml of blood.

Ethylene diamine tetraacetate (E.D.T.A):

• It may be used as 1 mg powder for 1 ml of blood.
• 1 drop of 10% solution will be adequate for 5 ml of blood.
• It should be not used for biochemical purposes.

Sodium citrate:

• It is prepared by mixing 3.8 g of sodium citrate to 100 ml of distilled water.
• 1 ml of this solution will be required 10 ml of blood.

Sodium fluoride:

• It may be used as 1mg per 1 ml blood.
• It prevents glycolysis and therefore suitable for blood sugar determination.

Heparin:

• It is made as 1% solution
• 1 ml is sufficient for 10 ml of blood.