Endospore staining

Moeller stain: It is a differential stain which differentiates endospores forming and non-endospores forming bacteria. It is used to presence and location of endospores. Moeller stain uses carbol fuschin(red), acid-alcohol and methylene blue.

Steps:

• Heat fix sample to the slide
• Add carbol fuchsin. Then steam slide or add detergent.
• Rinse with acid alcohol.
• Add methylene blue as a counter stain.