Culture media
Artificial culture media constitutes the food materials for the cultivation of microorganisms, which provides the in- vivo composition and conditions for the successful growth of bacteria, fungi and parasites in-vitro.
classification
- Based on consistency (state):
- Liquid(no agar) : peptone water, nutrient broth
- Semi-solid(1-1.5 % agar): nutrient agar stabs(TSI media)
- Solid(2.5% agar): blood agar, serum agar
2 . Based on oxygen requirement:
- Aerobic media: cultivation of aerobic organisms
- Anaerobic media: cultivation of anaerobic organisms. Eg. Robertson’s cooked meat media, peptone infusion broth.
3 . Sabauround’s dextrose agar: use for the cultivation of fungi.
4 . Simple media:
- It consists of only basic necessities and used for simply growth.
- Peptone water, nutrient broth, nutrient agar
- Peptone water(1% peptone+0.5%NaCl+100 ml water)
- Nutrient broth(peptone water+1%meat extract)
- Nutrient agar(nutrient broth+2% agar)
- Used to grow non-fastidious microorganisms
5 . Complex media(special media):
- Used to grow specific bacteria and constitute specific ingredients
- Have added ingredients for special purpose for bringing out certain properties or for providing special nutrients for the growth of bacterium under study.
- Enriched media, differential media, enrichment media, selective media, sugar media, transport media.