Course Content
An over view of biotechnology – history, process and product
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Some Terminologies
Introduction
HISTORY OF BIOTECHNOLOGY
Scope and importance of biotechnology
Agriculture, Industrial, Environmental and Healthcare fields of biotechnology
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Application of industrial biotechnology
Principles of immunology
Agricultural biotechnology:
Genetic engineering and gene cloning
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Introduction
Restriction enzyme
GENE CLONING VECTORS
Cloning and expression vectors
Classes of plasmids
Transferring genes into plants and animal cells
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Agrobacterium tumefaciens: An important vector for genetic engineering
Transformation
Agrobacterium tumefaciens mediated transformation
Other methods of transformation
Application of genetic engineering
Transgene silencing
Cell and tissue culture
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Plant tissue culture
Meristem Culture
Micropropagation
Embryo culture
ANTHER/POLLEN CULTURE
Protoplast culture
Callus culture
Suspension culture
Somatic hybridization
Somaclonal variation
Wide hybridization
Distant hybridization
Methods of virus elimination ( or Production of disease-free plants)
Virus indexing
Germplasm Conservation
Gene Pool
Animal biotechnology
The concept, aim and, scope of biodiversity
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Some Terminologies
Biodiversity
Diversity, classification, and nomenclature of cultivated plants, weeds, microbes, and insect-pests
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Introduction
Factors affecting biodiversity
Database
Indexing biodiversity
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Biodiversity indexing
Genetic erosion
Sampling technique for biodiversity indexing
Centers of diversity of crops and wild genetic diversity
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Center of origin
Conservation of biodiversity
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Intellectual Property Rights (IPR)
Types of Intellectual Property Rights (IPR)
Scope and Importance of Biodiversity
Concept of Biodiversity Conservation
Practice in Biodiversity Conservation
Learn Introductory Biotechnology and Biodiversity with Rahul

Transformation

  • Stable introduction, integration of foreign gene in an organism and its subsequent expression.

7.11B: Bacterial Transformation - Biology LibreTexts

Steps

  1. Identification and isolation.
  2. Introduction
  3. Integration
  4. Selection and expression
  5. Regeneration
  6. Transmission (Progeny).

 

Methods of transformation

  1. Indirect method:
  2. Agrobacterium tumefaciens mediated:
  3. Direct method:
  4. Particle gun
  5. Electroporation
  6. Polyethylene glycol
  7. Micro injection
  8. Direct DNA Uptake by cells
  9. Ca-phosphate precipitation
  10. Liposome mediated DNA transfer
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