Course Content
Carbohydrate
0/12
Introduction
Classification of polysaccharide
Organisms contain a variety of hexose derivative
Physical and Chemical properties of monosaccharide
Acetal formation
Aldol condensation
Reducing and Non-reducing sugar
Ring structure of ribose and its importance
Biosynthesis of Lactose
Biosynthesis of Starch
Biosynthesis of sucrose
Lac Operon model in E. coli
Lipid
0/17
Introduction
Classification of lipid
Some naturally occurring Fatty acids
Reactivity of unsaturated fatty acids
Triacyl glycerol provides store energy and insulation
Lipids with specific biological activities
Biological role of lipid
Polysaturated Fatty acids
Types of Linolenic acid
Structural lipids in membranes
Some Lipids
Lipid as signals, Cofactors and pigments
Structure and function of pyridoxial phosphate ( Vit. B6)
Lipoproteins (Lipids + Proteins)
Reaction of β-oxidation of saturated FA
Biosynthesis of fatty acids
Metabolism of lipid
Nucleic Acids
0/11
Introduction
Structures of Purine and Pyrimidine
Nucleotide and Nucleic Acid Nomenclature
Phosphodiester linkages in the covalent backbone of DNA and RNA
Characteristics of DNA
Watson and Crick DNA Model
Introduction to RNA
General Properties of Nucleic Acids
Reactions promoted by radiations
Biosynthesis of DNA or semi-conservative replication of DNA
Transcription
Biochemistry and molecular logic of life
0/1
Biochemistry
Cell and its components
0/8
Cell
Structure and cell wall composition of bacteria E. coli ( Gram negative)
Plasma membrane of bacteria
Bacterial toxigenesis
Eukaryotic cell
Fluid mosaic model of plasma membrane
Structure of cell organelles
Plant cell wall
Aqueous system of cell
0/5
Buffers
Phosphate buffer system
Bicarbonate buffer system
Haemoglobin buffer system
Ionization of water
Reactions and Bioenergetics of cell
0/2
Biomolecules
Concept of standard free energy of reactions
Enzymes
0/5
Introduction
Classification of enzyme @ OTH LyLI
Mode of Action of enzyme / Mechanism of enzyme Action
pH and Temperature dependency of enzyme Activity
Michaelis Menten equation
Carbohydrate Metabolism
0/3
Introduction
Glycolysis
Metabolic fates of absorbed glucose
Common terminal Pathway of Metabolism
0/5
Kreb cycle or TCA cycle or citric acid cycle
Structure of FAD and FADH2
Structure of NAD+ and NADP+
Calvin cycle or C3 pathway
Metabolism of pyruvate
Amino acids
0/7
Essential Amino acids ( PVT TIM HALL)
Non-Essential Amino acid ( GAC TPS)
Amino acid metabolism
Transamination reaction
Biosynthesis of amino acids
Laboratory test of Amino Acids
Process of Amino acid metabolism
Proteins
0/3
Classification of protein
Structure of protein
Reaction to test protein in lab
Metabolism of protein
0/5
Biosynthesis of protein
Structure of ribosome
Nature of genetic code
Transfer RNA (tRNA)
Translation
Hormones
0/3
Structures of various plant hormones
Classes of hormones
Animal hormones
Secondary metabolism
0/3
Introduction
Purpose of secondary metabolites production in plants
Application of secondary metabolite
Biochemical techniques
0/8
Principle of spectrophotometer
Application of Spectrophotometer
Techniques of paper chromatography
Chromatographic paper
Detection of compounds on chromatogram
Principle of electrophoresis
Gel electrophoresis of DNA
Polymerase chain reaction (PCR)
Learn General Biochemistry with Rahul

Gel electrophoresis of DNA

  • Used to identify, separate and purify DNA fragments.
  • Location of DNA is identified by staining with ethidium bromide.

Gel electrophoresis | DNA Separation & Analysis | Britannica

Steps:

  • Agarose gels are cast by melting the agarose in the presence of desired buffer, until a clear, transparent solution is obtained.
  • The melted solution is then poured into a mold and allowed to harden.
  • Upon hardening, agarose form matrix.
  • When electric field is applied across the gel, DNA which is -vely charged migrate towards the anode.
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