Detection of compounds on chromatogram

a) Physical method:

• Chromatogram observed first in daylight and the position of colored substance is marked.
• Chromatogram is then observed on ultraviolet light.
• Substance appear as fluorescent spots with characteristics hues.

b) Chemical method:

• Chromatogram is put on horizontal sheet of filter paper and sprayed with fine mist of reagent side by side.
• The substance appear as fluorescent substance.

c) Biological method:

• Chromatogram is pressed on a Petri dish with a corresponding culture of microorganism.
• Amylase detected by spraying the chromatogram with starch solution and iodine solution.
• Starch doesn’t react with iodine.