Gel electrophoresis of DNA

• Used to identify, separate and purify DNA fragments.
• Location of DNA is identified by staining with ethidium bromide.

Steps:

• Agarose gels are cast by melting the agarose in the presence of desired buffer, until a clear, transparent solution is obtained.
• The melted solution is then poured into a mold and allowed to harden.
• Upon hardening, agarose form matrix.
• When electric field is applied across the gel, DNA which is -vely charged migrate towards the anode.