Principle of electrophoresis

• Electrophoresis is the movement of charged particles in an electric field.
• Protein carry both +ve and -ve charges depending on the medium i.e. acidic or basic.
• At iso-electric point, when the protein bears both +ve and -ve charges, it behave as neutral.
• pH> Isoelectric point, protein carry -ve charge and moves towards anode.
• The molecular mass of protein is determined from its mobility.